Protocols I use in my research, shared in the spirit of open science. These are meant to be explaining the procedure to beginners, with practical tips. Hence, these are less stringent than typical published protocols, focusing more on the reasoning of various steps. They are living documents and get updated as I learn more. Questions or suggestions? Email me.
Agarose pads are a simple way to hold bacteria still on a coverslip for microscopy while giving them nutrients from the pad. Video protocol: how to make agarose pads for imaging happy bacteria under the microscope: watch …
Pass filtered phage lysate (phages removed from bacteria after growth) once through a 100 kDa Amicon filter (in a benchtop centrifuge, not an ultracentrifuge) to exchange the media to PBS (pH 7.4). This helps get rid of …
This protocol is written for folks who are starting out phage work and don’t super care about getting the maximum yield (highest titer or concentration of phages). Once you carry out literature review, you might find …